Composite

Part:BBa_K4800038:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-Hsd36


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3365
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3347
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3353
    Illegal PstI site found at 3365
    Illegal AgeI site found at 2611
    Illegal AgeI site found at 2683
    Illegal AgeI site found at 3292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3549
    Illegal BsaI site found at 4617
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References